Compounds
Some inhibitors suitable for molecular tattoo are already available commercially, distributors include Bio-Connect Services BV , Waken B Tech Co.,Ltd. , Optopharma Ltd. and Axol Bioscience Ltd. .
Photoreactive myosin inhibitor, azidoblebbistatin
Reference: Azidoblebbistatin, a photoreactive myosin inhibitor by Miklós Képiró, Boglárka H. Várkuti, Andrea Bodor, György Hegyi, László Drahos, Mihály Kovács, András Málnási-Csizmadia in Proc Natl Acad Sci U S A. 2012 Jun 12; 109(24):9402-7. doi: 10.1073/pnas.1202786109 Pubmed link
In vivo application reference: Molecular tattoo: subcellular confinement of drug effects by Képiró M, Várkuti BH, Rauscher AA, Kellermayer MS, Varga M, Málnási-Csizmadia A. in Chem Biol. 2015 Apr 23;22(4):548-58. doi: 10.1016/j.chembiol.2015.03.013 Pubmed link
For data sheet and recommendations for usage click here.
Photostable myosin inhibitors suitable as controls for Molecular Tattoo are also available:
Non-phototoxic, stable and non-cytotoxic myosin inhibitor, para-nitroblebbistatin
Reference: Para-nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor by M. Képiró, B. H. Várkuti, L. Végner, G. Vörös, Gy. Hegyi, M. Varga, A. Málnási-Csizmadia in Angewandte Chemie Volume 53, Issue 31, July 28, 2014, DOI: 10.1002/anie.201403540 Pubmed link
For data sheet and recommendations for usage click here.
Para-nitroblebbistatin has already been used in independent research articles:
- Synaptic Vesicle Endocytosis Occurs on Multiple Timescales and Is Mediated by Formin-Dependent Actin Assembly by T. Soykan et al in Neuron 93, February 22, 2017, DOI:10.1016/j.neuron.2017.02.011 Link
- Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells by M. Chabaud et al in Nature Communications 6, June 25, 2015, DOI:10.1038/ncomms8526 Link
- Formin-generated actomyosin arcs proper T cell receptor microcluster movement at the immune synapse by S. Murugesan et al in J. Cell Biol. October 31, 2016, DOI: 10.1083/jcb.201603080 Link
- The growth determinants and transport properties of tunneling nanotube networks between B lymphocytes by Osteikoetxea-Molnar et al in Cell. Mol. Life Sci. April 28, 2016 DOI 10.1007/s00018-016-2233-y Link
- Evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation by Letelier et al in PNAS April 2, 2018 DOI 10.1073/pnas.1719885115 Link
- Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments by Spira et al in eLife November 6, 2017 DOI 10.7554/eLife.30867 Link
Highly soluble derivative, para-aminoblebbistatin
Reference: A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative by Boglárka H. Várkuti; Miklós Képiró; Ádám I. Horváth; László Végner; Szilvia Ráti; Áron Zsigmond; György Hegyi; Zsolt Lenkei; Máté Varga in Scientific Reports. 6: 26141. 2016 May 31 doi:10.1038/srep26141 PubMed Link
Para-aminoblebbistatin has been suggested as one of the best compounds to replace blebbistatin in a review by Roman et al.
Para-aminoblebbistatin enabled in vivo imaging of zebrafish heart by reducing motion artefacts in a research paper by Chantal J.M. van Opbergen et al.
For data sheet and recommendations for usage click here.
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